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KMID : 1007519970060040234
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Food Science and Biotechnology
1997 Volume.6 No. 4 p.234 ~ p.238
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Optimization for Elicitation in Immobilized Culture of Peppermint Cells
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Lee, Hyong Joo
Woo, Gun Jo/Ha, Won Ho
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Abstract
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To increase the productivity of peppermint oil in an immobilized culture of peppermint (Menthes piperita L.) cells, the effects of elicitation on oil production were investigated. Peppermint cells were immobilized in polyurethane foams of 5×5×5 ㎣ in volume. The immobilized peppermint cells resulted in about 70% of cell viability and 2.0 ㎎ of dry cell weight (DCW)/ foam during 30 days of cultivation. The characteristics of chitosan and pectinase as the elicitors for an immobilized culture of peppermint cells were compared. The optimum concentrations of chitosan and pectinase were 50 ㎎/ℓ and 15 U/ℓ, respectively, for a short term (one day) elicitor treatment. For a long term (30 day) cultivation with elicitation, 5 ㎎ chitosan/ℓ and 1.5 U pectinase/ℓ were selected as optimum treatment concentrations. The addition of chitosan to the culture medium increased specific oil productivity, however, the cell viability was decreased by elicitation, especially by the pectinase treatment. Under the optimum conditions of elicitation, 5 ㎎/ℓ of chitosan treatment throughout the culture period, productivity of peppermint oil was 287 ㎎/ℓ in a 30-day culture of immobilized peppermint cells and specific oil productivity reached 153 ㎎/g of DCW.
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